Intraperitoneal injection of cell-engineered pancreas in rats with experimental type i diabetes (preliminary results)

Creation of a bioartificial pancreas, including cell-engineered construct (CEC) formed from pancreatic islets (islets Langerhans) and biocompatible matrix mimicking the native microenvironment tissue, is one approaches to treatment type 1 diabetes mellitus (T1D). Objective: conduct preliminary in vivo studies functional efficacy intraperitoneal injection endocrine suspension rat an experimental T1D model. Materials methods . Tissue-specific scaffold was obtained by decellularization human fragments. The viability activity isolated with collagenase were determined. Experimental modeled low-dose streptozotocin incomplete Freund’s adjuvant into rats. rats intraperitoneally injected twice CEC (n = 2) or islet 1). Glucose levels blood urine assessed. Histological examination organs (pancreas kidneys) animals carried out. Results. After first injection, glucose gradually decreased all more than 47% initial values; follow-up day 24, level rose hyperglycemic values. repeated administration, 63.4% decrease glycemic observed 47.5% suspension. At week 5 experiment, increased animals. same time, index 62% lower islets. Conclusion. Allogeneic increase duration stable